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quickchange multisite-directed mutagenesis kit  (Agilent technologies)


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    Structured Review

    Agilent technologies quickchange multisite-directed mutagenesis kit
    Quickchange Multisite Directed Mutagenesis Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/multisite-quickchange+kit/pm38172512-289-7-11
    Average 90 stars, based on 1 article reviews
    quickchange multisite-directed mutagenesis kit - by Bioz Stars, 2026-10
    90/100 stars

    Images

    Related Articles

    Clone Assay:

    Article Title: miR-142-5p Disrupts Neuronal Morphogenesis Underlying Porcine Hemagglutinating Encephalomyelitis Virus Infection by Targeting Ulk1
    Article Snippet: Mutation of the miR-142-5p binding site was achieved using the Multisite-Quickchange kit (Stratagene, USA) according to the manufacturer's protocol.

    Article Title: Identification of miR-148a as a novel regulator of cholesterol metabolism
    Article Snippet: Point mutations in the seed region of the predicted miR-148a binding sites within the 3′ UTR of LDLR were generated using the Multisite-Quickchange Kit (Stratagene), according to the manufacturer’s protocol.

    Article Title: miR-27b inhibits LDLR and ABCA1 expression but does not influence plasma and hepatic lipid levels in mice
    Article Snippet: Point mutations in the seed region of the predicted miR-27b binding sites within the 3’UTR of LDLR, ABCA1 and LDLRAP1 were generated using the Multisite-Quickchange Kit (Stratagene), according to the manufacturer’s protocol.

    Article Title: Downregulation of MicroRNA-193b-3p Promotes Autophagy and Cell Survival by Targeting TSC1/mTOR Signaling in NSC-34 Cells
    Article Snippet: The TSC1 3′UTR mutations were introduced using the Multisite-Quickchange kit (Stratagene, CA, USA) according to the manufacturer’s protocol and cloned into the pMIR-REPORT luciferase vector (Ambion, USA), and the reconstituted plasmid was named pMUT.

    Luciferase:

    Article Title: miR-142-5p Disrupts Neuronal Morphogenesis Underlying Porcine Hemagglutinating Encephalomyelitis Virus Infection by Targeting Ulk1
    Article Snippet: Mutation of the miR-142-5p binding site was achieved using the Multisite-Quickchange kit (Stratagene, USA) according to the manufacturer's protocol.

    Article Title: Identification of miR-148a as a novel regulator of cholesterol metabolism
    Article Snippet: Point mutations in the seed region of the predicted miR-148a binding sites within the 3′ UTR of LDLR were generated using the Multisite-Quickchange Kit (Stratagene), according to the manufacturer’s protocol.

    Article Title: miR-27b inhibits LDLR and ABCA1 expression but does not influence plasma and hepatic lipid levels in mice
    Article Snippet: Point mutations in the seed region of the predicted miR-27b binding sites within the 3’UTR of LDLR, ABCA1 and LDLRAP1 were generated using the Multisite-Quickchange Kit (Stratagene), according to the manufacturer’s protocol.

    Article Title: Downregulation of MicroRNA-193b-3p Promotes Autophagy and Cell Survival by Targeting TSC1/mTOR Signaling in NSC-34 Cells
    Article Snippet: The TSC1 3′UTR mutations were introduced using the Multisite-Quickchange kit (Stratagene, CA, USA) according to the manufacturer’s protocol and cloned into the pMIR-REPORT luciferase vector (Ambion, USA), and the reconstituted plasmid was named pMUT.

    Plasmid Preparation:

    Article Title: miR-142-5p Disrupts Neuronal Morphogenesis Underlying Porcine Hemagglutinating Encephalomyelitis Virus Infection by Targeting Ulk1
    Article Snippet: Mutation of the miR-142-5p binding site was achieved using the Multisite-Quickchange kit (Stratagene, USA) according to the manufacturer's protocol.

    Article Title: Identification of miR-148a as a novel regulator of cholesterol metabolism
    Article Snippet: Point mutations in the seed region of the predicted miR-148a binding sites within the 3′ UTR of LDLR were generated using the Multisite-Quickchange Kit (Stratagene), according to the manufacturer’s protocol.

    Article Title: miR-27b inhibits LDLR and ABCA1 expression but does not influence plasma and hepatic lipid levels in mice
    Article Snippet: Point mutations in the seed region of the predicted miR-27b binding sites within the 3’UTR of LDLR, ABCA1 and LDLRAP1 were generated using the Multisite-Quickchange Kit (Stratagene), according to the manufacturer’s protocol.

    Article Title: Downregulation of MicroRNA-193b-3p Promotes Autophagy and Cell Survival by Targeting TSC1/mTOR Signaling in NSC-34 Cells
    Article Snippet: The TSC1 3′UTR mutations were introduced using the Multisite-Quickchange kit (Stratagene, CA, USA) according to the manufacturer’s protocol and cloned into the pMIR-REPORT luciferase vector (Ambion, USA), and the reconstituted plasmid was named pMUT.

    Mutagenesis:

    Article Title: miR-142-5p Disrupts Neuronal Morphogenesis Underlying Porcine Hemagglutinating Encephalomyelitis Virus Infection by Targeting Ulk1
    Article Snippet: Mutation of the miR-142-5p binding site was achieved using the Multisite-Quickchange kit (Stratagene, USA) according to the manufacturer's protocol.

    Article Title: Identification of miR-148a as a novel regulator of cholesterol metabolism
    Article Snippet: Point mutations in the seed region of the predicted miR-148a binding sites within the 3′ UTR of LDLR were generated using the Multisite-Quickchange Kit (Stratagene), according to the manufacturer’s protocol.

    Article Title: miR-27b inhibits LDLR and ABCA1 expression but does not influence plasma and hepatic lipid levels in mice
    Article Snippet: Point mutations in the seed region of the predicted miR-27b binding sites within the 3’UTR of LDLR, ABCA1 and LDLRAP1 were generated using the Multisite-Quickchange Kit (Stratagene), according to the manufacturer’s protocol.

    Article Title: Downregulation of MicroRNA-193b-3p Promotes Autophagy and Cell Survival by Targeting TSC1/mTOR Signaling in NSC-34 Cells
    Article Snippet: The TSC1 3′UTR mutations were introduced using the Multisite-Quickchange kit (Stratagene, CA, USA) according to the manufacturer’s protocol and cloned into the pMIR-REPORT luciferase vector (Ambion, USA), and the reconstituted plasmid was named pMUT.

    Binding Assay:

    Article Title: miR-142-5p Disrupts Neuronal Morphogenesis Underlying Porcine Hemagglutinating Encephalomyelitis Virus Infection by Targeting Ulk1
    Article Snippet: Mutation of the miR-142-5p binding site was achieved using the Multisite-Quickchange kit (Stratagene, USA) according to the manufacturer's protocol.

    Article Title: Identification of miR-148a as a novel regulator of cholesterol metabolism
    Article Snippet: Point mutations in the seed region of the predicted miR-148a binding sites within the 3′ UTR of LDLR were generated using the Multisite-Quickchange Kit (Stratagene), according to the manufacturer’s protocol.

    Article Title: miR-27b inhibits LDLR and ABCA1 expression but does not influence plasma and hepatic lipid levels in mice
    Article Snippet: Point mutations in the seed region of the predicted miR-27b binding sites within the 3’UTR of LDLR, ABCA1 and LDLRAP1 were generated using the Multisite-Quickchange Kit (Stratagene), according to the manufacturer’s protocol.

    Article Title: Downregulation of MicroRNA-193b-3p Promotes Autophagy and Cell Survival by Targeting TSC1/mTOR Signaling in NSC-34 Cells
    Article Snippet: The TSC1 3′UTR mutations were introduced using the Multisite-Quickchange kit (Stratagene, CA, USA) according to the manufacturer’s protocol and cloned into the pMIR-REPORT luciferase vector (Ambion, USA), and the reconstituted plasmid was named pMUT.

    Generated:

    Article Title: miR-142-5p Disrupts Neuronal Morphogenesis Underlying Porcine Hemagglutinating Encephalomyelitis Virus Infection by Targeting Ulk1
    Article Snippet: Mutation of the miR-142-5p binding site was achieved using the Multisite-Quickchange kit (Stratagene, USA) according to the manufacturer's protocol.

    Article Title: Identification of miR-148a as a novel regulator of cholesterol metabolism
    Article Snippet: Point mutations in the seed region of the predicted miR-148a binding sites within the 3′ UTR of LDLR were generated using the Multisite-Quickchange Kit (Stratagene), according to the manufacturer’s protocol.

    Article Title: miR-27b inhibits LDLR and ABCA1 expression but does not influence plasma and hepatic lipid levels in mice
    Article Snippet: Point mutations in the seed region of the predicted miR-27b binding sites within the 3’UTR of LDLR, ABCA1 and LDLRAP1 were generated using the Multisite-Quickchange Kit (Stratagene), according to the manufacturer’s protocol.

    Article Title: Downregulation of MicroRNA-193b-3p Promotes Autophagy and Cell Survival by Targeting TSC1/mTOR Signaling in NSC-34 Cells
    Article Snippet: The TSC1 3′UTR mutations were introduced using the Multisite-Quickchange kit (Stratagene, CA, USA) according to the manufacturer’s protocol and cloned into the pMIR-REPORT luciferase vector (Ambion, USA), and the reconstituted plasmid was named pMUT.



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